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Month: December 2017

Continuous cell lines commonly used for virus cultivation

December 20, 2017 Gaurab Karki 0

Continuous cell lines commonly used for virus cultivation Continuous Cell lines Viruses cultivated HeLa (Human epithelial cell line of cervical carcinoma) Respiratory syncytial virus, Adenovirus, […]

Mycoplasma: habitat, morphology, culture and biochemical characteristics

December 10, 2017 Gaurab Karki 0

Mycoplasma: habitat, morphology, culture and biochemical characteristics Mycoplasma are pleuromorphic smallest living organism, also known as Pleuropneumonia like organism (PPLO). Belongs to family Mycoplasmataceae Habitat: […]

Genus Streptococcus: habitat, morphology, culture and biochemical characteristics

December 9, 2017 Gaurab Karki 0

Genus Streptococcus: habitat, morphology, culture and biochemical characteristics The genus streptococcus include a large number of species of Gram +ve, catalase –ve cocci arranged in […]

Virulence factors and streptococcal infections

December 9, 2017 Gaurab Karki 0

Virulence factors and streptococcal infections Virulence factors of streptococcus: Capsular hyaluronic acid Group specific polysaccharide antigen Type specific antigen Protein F and G Exotoxins Hemolysin: […]

Dengue pathogenesis, clinical manifestation, lab diagnosis and treatment

December 8, 2017 Gaurab Karki 0

Dengue pathogenesis, clinical manifestation, lab diagnosis and treatment Pathogenesis of Dengue: Dengue virus after entering in the body invades the local macrophages and multiply there. […]

Dengue virus: structure, serotypes and mode of transmission

December 8, 2017 Gaurab Karki 0

Dengue virus: structure, serotypes and mode of transmission Introduction Dengue is an arthropod borne virus belongs to family Flaviviridae and genus Flavivirus. Dengue virus is […]

Western blotting technique: principle, procedure and application

December 4, 2017 Gaurab Karki 0

Western blot  Principle: Western blotting technique is used for identification of particular protein from the mixture of protein. In this method labelled antibody against particular […]

Southern Blotting: principle, procedure and application

December 3, 2017 Gaurab Karki 0

Southern Blotting  Principle: Southern blotting is an example of RFLP (restriction fragment length polymorphism). It was developed by Edward M. Southern (1975). Southern blotting is […]

Polymerase chain reaction (PCR): Principle, procedure or steps, types and application

December 2, 2017 Gaurab Karki 0

Polymerase chain reaction (PCR): Principle, procedure or steps, types and application Principle: Polymerase chain reaction is method for amplifying particular segments of DNA. It is […]

Role of CD4+ cells (T-lymphocytes, macrophages and monocytes) and lymphoid organs in HIV infection

December 1, 2017 Gaurab Karki 0

Role of CD4+ cells (T-lymphocytes, macrophages and monocytes) and lymphoid organs in HIV infection 1. Role of CD4+ T-lymphocytes in HIV infection: CD4+ T-cells are […]

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