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Month: May 2018

Nipah virus: Structure and genome, mode of transmission, Pathogenesis, Symptoms, prevention and treatment

May 26, 2018 Gaurab Karki 0

Nipah virus: Structure and genome, mode of transmission, Pathogenesis, Symptoms, prevention and treatment  Structure and genome of Nipah virus: Nipah virus ( NiV) is a […]

Benedict’s Test: Objective, Principle, Reagents, Procedure and Result

May 19, 2018 Gaurab Karki 0

Benedict’s Test: Objective, Principle, Reagents, Procedure and Result Objective: to detect reducing sugar ( carbohydrate having free aldehyde or ketone functional group) Principle: The Reducing […]

Fatty acids: Definition and Types of fatty acid

May 19, 2018 Gaurab Karki 0

Fatty acids: Definition and Types of fatty acid Fatty acids are carboxylic acids with hydrocarbon chain ranging from 4 to 36 caarbon atom ie. C4 […]

Barfoed’s Test: Objective, Principle, Reagents, Procedure and Result

May 12, 2018 Gaurab Karki 0

Barfoed’s Test: Objective, Principle, Reagents, Procedure and Result Objective: to detect reducing sugar to distinguish monosaccharides from reducing disaccharides Principle of Barfoed’s test: Barfoed’s test […]

DNA extraction from Gram Positive bacteria (Staphylococcus aureus): materials required and protocol

May 5, 2018 Gaurab Karki 1

DNA extraction from Gram Positive bacteria (Staphylococcus aureus): materials required and protocol Materials required: Overnight culture of Staphylococcus aureus in LB TE buffer ( 10mM […]

DNA extraction from E. coli: materials required and protocol

May 5, 2018 Gaurab Karki 0

DNA extraction from E. coli: Materials required: Overnight culture of coli in LB TE buffer ( 10mM tris.Cl, 1mM EDTA, pH 8.0) 10% SDS 1:1 […]

  Molisch’s Test: Objectives, Principle, Reagents, Procedure and Result

May 1, 2018 Gaurab Karki 0

Molisch’s Test: Objectives, Principle, Reagents, Procedure and Result Objective: to identify carbohydrate from other biomolecules Principle of Molisch’s test: Molisch’s test is a general test […]

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