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Month: April 2020

Cetrimide test: Principle, Requirements, Procedure and Result interpretation

April 29, 2020 Gaurab Karki 0

Principle: Cetrimide is a quaternary ammonium, cationic detergent that is toxic to most bacteria, except Pseudomonas aeruginosa and a few other bacteria. Nitrogen and phosphorus […]

CAMP test: Principle, Requirements, Procedure and Result interpretation

April 28, 2020 Gaurab Karki 0

Principle: This test was first described by Christie, Atkins and Munch-Petersen (1944) and the test name CAMP is derived from the initials of the author’s name. […]

Isolation and Identification of Brucella spp from clinical samples

April 26, 2020 Gaurab Karki 0

Brucella spp Brucella is a fastidious, aerobic, small, gram-negative coccobacillus that is slow growing and difficult to isolate. Four species of Brucella are recognized causing […]

Acridine orange staining: principle, requirements, procedure, result interpretation and limitations

April 26, 2020 Gaurab Karki 0

Principle: Acridine orange is a fluorochromatic dye which binds to nucleic acids of bacteria and other cells that causes deoxyribonucleic acid (DNA) to fluoresce green […]

Capsule staining: Principle, Reagents, Procedure and Result

April 26, 2020 Gaurab Karki 0

Principle A capsule is non-ionic and very delicate structure that is secreted by the cell and that surrounds and adheres to the cell wall. It […]

Transposable elements in eukaryotes

April 20, 2020 Gaurab Karki 0

There are many different types of transposons in eukaryotes which vary in size, structure, composition and behavior. All the eukaryotic transposons have basic characteristics such […]

Transposable elements in Prokaryotes (Bacteria)

April 20, 2020 Gaurab Karki 0

There are two main type of transposable elements in bacteria having different size and structure. They are; Insertion sequences (IS elements) Prokaryotic Transposons (Tn): Composite […]

Transposable elements: characteristics and mechanisms of transposition

April 20, 2020 Gaurab Karki 0

Transposable elements are the specific sequence of DNA which is mobile in nature and have capacity to transport from one position to another position in […]

T-cell receptor (TCR): structure, role and TCR-CD3 complex

April 19, 2020 Gaurab Karki 0

Structure of T-cell receptor (TCR): The domain structure of αβ and Ƴ𝛿 TCR heterodimers are stringly similar to those of immunologlobins; thus they are classified […]

Application of antisense technology

April 5, 2020 Gaurab Karki 0

Antisense technology is used successfully in two general areas, especially viral infections and cancers. However, different newer field have been emerging as the antisense technology […]

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