Somaclonal variation: Basis, Applications and limitations
Somaclonal variation The term somaclonal variation by Larkin and Scowcroft (1981) was given for the variability generated by the use of a tissue culture cycle. […]
Articles by Gaurab Karki
Apoptosis (Programmed cell death)
Cell death : One of the weirdest fate of the cell is the programmed cell death. Despite being odd fate it is essential for the […]
Novobiocin susceptibility test: Principle, Procedure and Results interpretations
Principle: After E. coli, Staphylococcus saprophyticus is the second most causative organism for the Urinary tract infections (UTIs) in sexually active young women. Laboratory identification […]
Cytoplasmic hybrid (Cybrid)-applications and limitations
Cybrids Somatic hybrids can be obtained where nucleus is derived from one parent and cytoplasm is derived from both the parents, thus resulting cytoplasmic hybrids, […]
Protoplast fusion: Methods and mechanism
Protoplast fusion/Somatic fusion: Protoplast fusion is a physical method of fusion of somatic cells from different plant to form hybrid. Mixing of protoplasts of two […]
Protocol for Protoplast fusion
Protoplast fusion by Polyethylene glycol (PEG) method Material: Green leaf (mesophyll) and cultured cells (albino) of N. tabacum PEG fusion solution contains: 22.5% (w/v) PEG […]
Protocol for protoplast isolation
Requirements-materials and reagents Plant materials- leaf of Nicotiana tabacum Cell and protoplast washing (CPW) solution 10% sodium hypochlorite Tween 20 13% mannitol-inorganic salts Enzyme mixture: […]
Protoplast Isolation- mechanical and enzymatic methods
Protoplast Isolation: The protoplast, also termed as naked plant cell refers to all the components of a plant cell excluding the cell wall. The term […]
Cryopreservation: Principle, Process, limitations and precautions
Principle of Cryopreservation: Cryopreservation is a process of preserving or storing cells, tissues, organs or any other biological materials from any potential damage by maintaining the […]
In vitro methods of germplasm conservation
In vitro conservation of plant germplasm In vitro method is an advanced technology of ex situ conservation for the preservation of genetic materials. In vitro […]