Partition chromatography-principle, instrumentation and application
Introduction First developed by Martin and Syange in 1941, illustrated the technique for the separation of amino acids. Their basic was to use liquid phase […]
Biochemistry
Blood Urea: normal value, clinical significance and methods of estimation
Introduction: Urea is the chief nitrogenous waste formed during protein metabolism in man. It is devised principally from the amino groups of amino acids. Liver […]
Thin layer Chromatography (TLC)-Principle and procedure
Principle of thin layer chromatography It is similar to paper chromatography except that a thin (0.25mm) layer of some inert material such as Al2O3, MgO, […]
Blood Glucose: normal value, clinical significance and methods of estimation
Introduction For all practical purposes, glucose is the only sugar that is present in the blood. Glucose is absorbed by the body cells and is […]
Lipid profile of blood-Normal value, significance and methods of estimation
Introduction: Lipids are organic substances that contain mostly carbon and hydrogen and some oxygen. A number of lipids also contain nitrogen and phosphorus. Lipids in […]
Paper chromatography – Principle, Procedure, types and applications
Principle of paper chromatography: This technique is a type of partition chromatography in which the substances are distributed between two liquids, i.e., one is the […]
Oxidative phosphorylation: Electron transport chain and ATP synthesis
Oxidative phosphorylation: Reducing equivalent NADH, FADH2 generated during glycolysis and the link between glycolysis and Kreb’s cycle are used to synthesize ATP by a process […]
Vitamin B complex: Structure, coenzyme forms and Biological roles
Vitamins are organic compound which in small amounts is required to perform specific biological function for normal maintenance and optimum growth of body. Vitamin B […]
Vitamin C (Ascorbic acid): Properties, biosynthesis, biological functions and deficiency
Vitamin C is well known for its ability to treat Scurvy. It is water soluble vitamin, also know as ascorbic acid. Vitamin C has three […]
Vitamin E (Tocopherol): Biosynthesis, Biological roles and Deficiency
Vitamin E is also termed as anti-sterility vitamin It is a very powerful biological antioxidant. It is a common term for a group of closely […]