O-Nitrophenyl-β-D-Galactopyranoside (ONPG) test: Principle, Procedure and Results
Principle of ONPG test: Lactose is a disaccharide of glucose and galactose which is connected by a β-galactoside bond. Lactose fermenting bacteria consist of both […]
Biology Practical
L-Pyrrolidonyl Arylamidase (PYR) test: Principle, Requirements, Procedure and Results
Principle of PYR test: L-Pyrrolidonyl Arylamidase (PYR) test is one of the rapid tests employed for the identification of group a beta-hemolytic Streptococci and Enterococci […]
Decarboxylase test-Principle, Procedure and Results
Principle of Decarboxylase test: Decarboxylases are a group of enzymes which acts by hydrolyzing an amino acid to form an amine. Decarboxylase test is mainly […]
Leucine Amino Peptidase (LAP) test
Principle: Leucine Amino Peptidase (LAP) test is one of the rapid tests which is employed for the detection of the enzyme leucine amino peptidase. The […]
Microdase (Modified Oxidase) test: Principle, Procedure and Results interpretation
Principle: Microdase test is also termed as modified oxidase test. It is one of the rapid tests employed for the differentiation between Staphylococcus and Micrococcus […]
Novobiocin susceptibility test: Principle, Procedure and Results interpretations
Principle: After E. coli, Staphylococcus saprophyticus is the second most causative organism for the Urinary tract infections (UTIs) in sexually active young women. Laboratory identification […]
Acetate utilization test: Principle, media composition, Procedure and Results
Principle of Acetate utilization test: Acetate agar is employed to test an organism’s ability to utilize acetate. The medium consists of sodium acetate as the […]
Bacitracin test: Principle, Requirements, Procedure, Results interpretations, Limitations
Principle: Bacitracin is the antibiotic produced by Bacillus licheniformis and this drug interferes the formation of peptidoglycan, a major component of bacterial cell wall. Bacitracin blocks bactoprenol […]
Bile solubility test: Principle, Procedure, and Result
Principle of Bile solubility test : The bile solubility test is employed to distinguish Streptococcus pneumoniae from alpha-haemolytic Streptococcus spp. The basis of bile solubility […]
Hippurate hydrolysis test: Principle, Procedure, Result interpretations and Limitations
Principle of Hippurate hydrolysis test : Hippurate hydrolysis test is performed for the identification of Campylobacter jejuni, Listeria monocytogenes, Gardnerella vaginalis, and Streptococcus agalactiae. The […]