Protocol for Protoplast fusion
Protoplast fusion by Polyethylene glycol (PEG) method Material: Green leaf (mesophyll) and cultured cells (albino) of N. tabacum PEG fusion solution contains: 22.5% (w/v) PEG […]
Biotechnology practical
Protocol for protoplast isolation
Requirements-materials and reagents Plant materials- leaf of Nicotiana tabacum Cell and protoplast washing (CPW) solution 10% sodium hypochlorite Tween 20 13% mannitol-inorganic salts Enzyme mixture: […]