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Biotechnology

Protoplast fusion: Methods and mechanism

Protoplast fusion: Methods and mechanism

June 25, 2020 Gaurab Karki 0

Protoplast fusion/Somatic fusion: Protoplast fusion is a physical method of fusion of somatic cells from different plant to form hybrid. Mixing of protoplasts of two […]

Protoplast Isolation- mechanical and enzymatic methods

June 24, 2020 Gaurab Karki 0

Protoplast Isolation: The protoplast, also termed as naked plant cell refers to all the components of a plant cell excluding the cell wall. The term […]

Application of antisense technology

April 5, 2020 Gaurab Karki 0

Antisense technology is used successfully in two general areas, especially viral infections and cancers. However, different newer field have been emerging as the antisense technology […]

Antisense technology and gene silencing

April 5, 2020 Gaurab Karki 0

Antisense technology: Antisense technology is a recent approach to specific modification or inhibition of gene expression in vitro or in vivo. It is a tool […]

Human insulin production by genetic engineering

October 27, 2018 Gaurab Karki 0

Human insulin production by genetic engineering Insulin is a hormone produced by β-cells of islets of Langerhans of pancreas. It was discovered by sir Edward […]

Brewing; beer production process

October 24, 2018 Gaurab Karki 0

Brewing; beer production process Brewing is the process of production of malt beverages. Beers, ale and lagers are the main malt beverages produced by a […]

Enzymes used in recombinant DNA technology

November 19, 2017 Gaurab Karki 0

Enzymes used in recombinant DNA technology DNA ligase Reverse transcriptase Restriction endonuclease Terminal transcriptase Nuclease DNA polymease Ribonuclease-H Alkaline phosphatase Polynucleotide kinase 1. DNA ligase: […]

Production of human growth hormone (hGH) by recombinant DNA technology

November 18, 2017 Gaurab Karki 0

Production of human growth hormone (hGH) by recombinant DNA technology The gene for human growth hormone (hGH) is isolated from human pituitary gland. Insertion of […]

Cloning vector-characteristics and types

June 5, 2017 Gaurab Karki 0

Cloning vector-characteristics and types Cloning vector Cloning vector is a small DNA molecule capable of self-replication inside the host cell. Cloning vector is used for […]

Gene cloning-Steps involved in gene cloning

June 3, 2017 Gaurab Karki 0

Gene cloning- Steps involved in gene cloning Gene cloning Gene cloning involves separation of specific gene or DNA fragments from a donor cell, attaching it […]

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