Protoplast fusion: Methods and mechanism
Protoplast fusion/Somatic fusion: Protoplast fusion is a physical method of fusion of somatic cells from different plant to form hybrid. Mixing of protoplasts of two […]
Biotechnology
Protoplast Isolation- mechanical and enzymatic methods
Protoplast Isolation: The protoplast, also termed as naked plant cell refers to all the components of a plant cell excluding the cell wall. The term […]
Application of antisense technology
Antisense technology is used successfully in two general areas, especially viral infections and cancers. However, different newer field have been emerging as the antisense technology […]
Antisense technology and gene silencing
Antisense technology: Antisense technology is a recent approach to specific modification or inhibition of gene expression in vitro or in vivo. It is a tool […]
Human insulin production by genetic engineering
Human insulin production by genetic engineering Insulin is a hormone produced by β-cells of islets of Langerhans of pancreas. It was discovered by sir Edward […]
Brewing; beer production process
Brewing; beer production process Brewing is the process of production of malt beverages. Beers, ale and lagers are the main malt beverages produced by a […]
Enzymes used in recombinant DNA technology
Enzymes used in recombinant DNA technology DNA ligase Reverse transcriptase Restriction endonuclease Terminal transcriptase Nuclease DNA polymease Ribonuclease-H Alkaline phosphatase Polynucleotide kinase 1. DNA ligase: […]
Production of human growth hormone (hGH) by recombinant DNA technology
Production of human growth hormone (hGH) by recombinant DNA technology The gene for human growth hormone (hGH) is isolated from human pituitary gland. Insertion of […]
Cloning vector-characteristics and types
Cloning vector-characteristics and types Cloning vector Cloning vector is a small DNA molecule capable of self-replication inside the host cell. Cloning vector is used for […]
Gene cloning-Steps involved in gene cloning
Gene cloning- Steps involved in gene cloning Gene cloning Gene cloning involves separation of specific gene or DNA fragments from a donor cell, attaching it […]