Immunostaining of developing Embryos of Drosophila
Embryonic development of Drosophila A series of rapid nuclear cycles takes place during the first few hours of Drosophila development, some of cycles take as […]
Genetics practical
Immunofluorescent staining of Drosophila Larval Neuroblasts
Larval neuroblasts The larval brain is the tissue of choice for the study of cell division during late development. Most mitotic mutants of Drosophila have […]
Fluorescent in-situ Hybridization to Diploid Cells
Protocol of in-situ hybridization In situ hybridization to diploid cells serves in mapping and characterizing the behaviour of heterochromatic sequences of Drosophila, which are both […]
Preparation of Polytene Chromosomes of Drosophila for In-Situ Hybridization
Polytene Chromosomes preparation Dissect out salivary glands from third-instar larvae in 0.7% NaCl. Transfer the salivary glands to a drop of 45 % acetic acid […]
Fluorescent in-situ hybridization: advantages, limitations and preparation of probe
Modified in-situ hybridization The modification is seen in the detection system, which is based on a fluorescence signal instead of the coloured precipitate resulted by […]
DNA extraction from Gram Positive bacteria (Staphylococcus aureus): materials required and protocol
DNA extraction from Gram Positive bacteria (Staphylococcus aureus): materials required and protocol Materials required: Overnight culture of Staphylococcus aureus in LB TE buffer ( 10mM […]
DNA extraction from E. coli: materials required and protocol
DNA extraction from E. coli: Materials required: Overnight culture of coli in LB TE buffer ( 10mM tris.Cl, 1mM EDTA, pH 8.0) 10% SDS 1:1 […]