Chromosomal theory of inheritance
Chromosomal theory of inheritance In 1902 and 1903, Sutton and Boveri published independent papers proposing the chromosome theory of inheritance. According to Chromosomal theory of inheritance, […]
Genetics
Human insulin production by genetic engineering
Human insulin production by genetic engineering Insulin is a hormone produced by β-cells of islets of Langerhans of pancreas. It was discovered by sir Edward […]
Transduction: generalized and specialized transduction
Transduction: generalized and specialized transduction Transduction is a method of gene transfer in bacteria from donor to recipient using bacteriophage. In transduction at first bacteriophage […]
High frequency recombination (Hfr) cell conjugation and F-prime (F’) cell
High frequency recombination (Hfr) cell conjugation and F-prime (F’) cell Hfr cell When F-plasmid (sex factor) integrated with chromosomal DNA then such bacteria is known […]
Bacterial Conjugation: steps and mechanism of transfer of plasmid from donor to recipient cell
Bacterial Conjugation: steps and mechanism of transfer of plasmid from donor to recipient cell Conjugation in bacteria is a process in which plasmids are transferred […]
Enzymes used in recombinant DNA technology
Enzymes used in recombinant DNA technology DNA ligase Reverse transcriptase Restriction endonuclease Terminal transcriptase Nuclease DNA polymease Ribonuclease-H Alkaline phosphatase Polynucleotide kinase 1. DNA ligase: […]
Bacterial Transformation: Competence in gram positive and gram negative bacteria
Transformation in Bacteria Transformation is the process of introduction of derived DNA fragments from a donor bacteria into a recipient bacteria. It is one of […]
Sanger’s method of gene sequencing
Sanger’s method of gene sequencing Sanger’s method of gene sequencing is also known as dideoxy chain termination method. It generates nested set of labelled fragments […]
Difference between Prokaryotic and Eukaryotic translation
Difference between Prokaryotic and Eukaryotic translation Translation Prokaryotes Eukaryotes Initiation factor Three (IF3, IF2, IF1) Nine (eIF4F complex; eIF4E, eIF4G,eIF4A) Ribosome 30S and 50S 40S […]
Translation in prokaryotes
Translation in Prokaryotes It is the process of synthesis of protein by encoding information on mRNA. Protein synthesis requires mRNA, tRNA, aminoacids, ribosome and enzyme […]