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Microbiology practical

Bacitracin test: Principle, Requirements, Procedure, Results interpretations, Limitations

June 10, 2020 Gaurab Karki 0

Principle: Bacitracin is the antibiotic produced by Bacillus licheniformis and this drug interferes the formation of peptidoglycan, a major component of bacterial cell wall.  Bacitracin blocks bactoprenol […]

Bile solubility test: Principle, Procedure, and Result

June 8, 2020 Gaurab Karki 0

Principle of Bile solubility test : The bile solubility test is employed  to distinguish Streptococcus pneumoniae from alpha-haemolytic Streptococcus spp. The basis of bile solubility […]

Hippurate hydrolysis test: Principle, Procedure, Result interpretations and Limitations

June 7, 2020 Gaurab Karki 0

Principle of Hippurate hydrolysis test : Hippurate hydrolysis test is performed for the identification of Campylobacter jejuni, Listeria monocytogenes, Gardnerella vaginalis, and Streptococcus agalactiae. The […]

MRS Broth test: Principle, Composition, Procedure, and Results interpretation

June 6, 2020 Gaurab Karki 0

Principle: MRS broth is a medium for the cultivation of lactobacilli and is specifically useful for a number of fastidious strains which is unable to […]

Optochin susceptibility test: Principle, Procedure and Results interpretation

June 6, 2020 Gaurab Karki 0

Principle of Optochin susceptibility test: Streptococcus pneumoniae is found generally on respiratory tract and has a hemolytic pattern identical to other alpha-hemolytic streptococci and lactobacilli. […]

4-Methylumbelliferyl-β-D-Glucuronide (MUG) Test

June 5, 2020 Gaurab Karki 0

Principle of MUG test: About 97% strains of Escherichia coli and organisms as such Salmonella, Shigella, and Yersinia are capable of producing enzyme β-glucuronidase. MUG […]

Salmonella-Shigella Agar test: Principle, Composition, Procedure, Results interpretation

June 3, 2020 Gaurab Karki 0

Principle: Salmonella-shigella (SS) agar is generally employed for the selective isolation and differentiation of Salmonella and Shigella in both clinical and nonclinical samples. However, SS […]

Satellitism test for identification of Haemophilus influenzae

June 3, 2020 Gaurab Karki 0

Principle: Haemophilus spp. typically grows on chocolate agar as smooth, flat or convex buff, or slightly yellow colonies. Chocolate agar provides hemin (X factor) and […]

Phenylalanine deaminase test: Principle, Requirements, Procedure and Results interpretations

June 2, 2020 Gaurab Karki 0

Principle: The phenylalanine deaminase (PDA) test is employed to differentiate among the urea- positive gram-negative bacilli on the basis of the ability of the microorganisms […]

Malonate test: Principle, Requirements, Procedure, and Result interpretations

June 2, 2020 Gaurab Karki 0

Objective: To check whether the organism is capable of utilizing malonate as sole source of carbon and energy for its growth. To categorize organisms on […]

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