Translation |
Prokaryotes |
Eukaryotes |
Initiation factor |
Three (IF3, IF2, IF1) |
Nine (eIF4F complex; eIF4E, eIF4G,eIF4A) |
Ribosome |
30S and 50S |
40S and 60s |
Shinedalgarno sequence at 5’ end |
Present (5’ UAAGGAGG3’). |
absent |
Poly A tail at 3’end |
absent |
present |
Elongation factor |
EF-TU, EF-TS, EF-G-GTP |
eEF1α, eEF1βϒ,eEF2 |
Release factors |
Three (RF1, RF2 and RF3) |
One (eRF) |
Chain initiating aminoacid |
N-formyl methionine |
methionine |
mRNA |
Polycistonic |
Monocistonic |
Stability of mRNA |
unstable |
Quite stable |
Rate of translation |
Faster process; adds 20 aminoacids per second |
Slower process; adds one aminoacid per cecond |
Placement of AUG codon P-site |
By shinedalgarno sequence |
Scanning of mRNA |