Protocol for protoplast isolation
Requirements-materials and reagents Plant materials- leaf of Nicotiana tabacum Cell and protoplast washing (CPW) solution 10% sodium hypochlorite Tween 20 13% mannitol-inorganic salts Enzyme mixture: […]
Requirements-materials and reagents Plant materials- leaf of Nicotiana tabacum Cell and protoplast washing (CPW) solution 10% sodium hypochlorite Tween 20 13% mannitol-inorganic salts Enzyme mixture: […]
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